-Cell Lipases and Insulin Secretion

Lipases in the pancreatic β-cell: implications for insulin secretion

Lipids have been implicated inβ-cell stimulus–secretion coupling. In such a role, lipases inβ-cellswould be required to generate lipid coupling factors. We have shown previously that glucose stimulates lipolysis in rodent islets. In addition, lipolysis and diacylglycerol lipase activity in islets are abolished by orlistat, an irreversible lipase inhibitor with a broad specificity for substrates...

Secretion of Extracellular Lipases by Cundidu ZipoZyticu

Lipolytic activity was associated with Candida lipolytica in several early studies (Long, 1936; Long & Hammer, 1938, 1939), and the influence of culture conditions on lipase production has been described by Peters & Nelson (1948). Studies on the intracellular lipases (Lloyd et al., 1971) indicated that C. lipoiytica grown in liquid media with glucose as a carbon source contained five distinct l...

The Cell Physiology of Biphasic Insulin Secretion.

Glucose-stimulated insulin secretion consists of a transient first phase followed by a sustained second phase. Diabetes (type II) is associated with abnormalities in this release pattern. Here we review the evidence that biphasic insulin secretion reflects exocytosis of two functional subsets of secretory granules and the implications for diabetes.

Lipases in heart and the effect of insulin

We have measured a-v differences across adipose and muscle tissues before and after a glucose load. T h e time course for the uptake of glucose by muscle is consistent with it being insulin sensitive; that for adipose tissue suggests it is not insulin sensitive. T h e glucose uptake by adipose tissue is quantitatively small, so adipose tissue lactate production can be only a small fraction of t...

Insulin Secretion and Actions